Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (2): 76-080.doi: 10.3969/j.issn.1004-616x.1998.02.004

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STUDIES ON THE GENOTOXICITY AND INHIBITORY EFFECTON GAP JUNCTION INTERCELLULAR COMMUNICATION OF BILE ACID

Liang Lixin et al.   

  1. Department of Toxicology , Second Military Medical University S hanghai , 200433
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-03-30 Published:1998-03-30

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Abstract: The genotoxicities of Deoxycholic acid and Lithocholtic acid were tested by Ames test and chromosome aberration test in vit ro. The malignent transformation effect of Deoxycholic acid and Lithocholic acid on NIH/ 3T3 cells was observed , The inhibitory effect of Deoxycholic acid and Lithocholic acid on gap junction intercellular communication ( GJ IC) in NIH/ 3T3 cells was investigated with Scrap Loading/ Dye Transfer test (SLDT) . The result s indicated that neither Deoxycholic acid nor Lithocholic acid has gene mutation , chromosome aberration and cell malignant t ransformation in vit ro , Therefore , it did not demonst rate that bile acid is a genotoxic carcinogen. But result showed that both Deoxycholic acid and Lithocholic acid could inhibit NIH/ 3T3 cells GJ IC obviously. It means that bile acid may play a role as promoter in carcinogenesis.

Key words: Deoxycholic acid, Lithocholic acid, Ames test, chromosome aberration, cell malignant t ransfomation, gap junction intercellular communication( GJ IC)